Journal: Toxicology

Article Title: Tumour necrosis factor-α (TNF-α) enhances dietary carcinogen-induced DNA damage in colorectal cancer epithelial cells through activation of JNK signaling pathway

doi: 10.1016/j.tox.2021.152806

Figure Lengend Snippet: CYP1A1/1B1 gene expression in HCT116 . (A) Cells were treated with TNF-α dose range of (0.01-100 pg/mL) over 72 h. (B ) cells co- treated with BaP and PhIP. Gene expression were measured by RT-qPCR. Data are shown as fold change. Significance was calculated using one-way ANOVA with a Dunnett test (GraphPad Prism 8). The data are presented as SEM from three independent cultures.

Article Snippet: Significant differences (P < 0.05) were determined using one-way analysis of variance (ANOVA) followed by a Dunnet post test (Graphpad Prism 8, GraphPad software Inc., La Jolla, CA., USA).

Techniques: Gene Expression, Quantitative RT-PCR

Journal: Toxicology

Article Title: Tumour necrosis factor-α (TNF-α) enhances dietary carcinogen-induced DNA damage in colorectal cancer epithelial cells through activation of JNK signaling pathway

doi: 10.1016/j.tox.2021.152806

Figure Lengend Snippet: Genotoxicity in HCT116 cells. DNA damage measured by Micronucleus frequency in HCT116 p53-/- and HCT116 p53+/+ cells induced by (A ) HCT116 p53-/- treated with TNF-α dose range of (0.01-100 pg/mL) and (B ) HCT116 p53-/- treated with BaP dose range of (0.1, 1,10μM) and PhIP dose range of (1-100μM), ( C ) HCT116 p53+/+ treated with BaP and PhIP. Statistically Significant differences are shown for comparisons between treated samples Vs. vehicle (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Significance was calculated using one-way ANOVA with a Dunnett test (GraphPad Prism 8). The data are presented as SEM from three independent cultures.

Article Snippet: Significant differences (P < 0.05) were determined using one-way analysis of variance (ANOVA) followed by a Dunnet post test (Graphpad Prism 8, GraphPad software Inc., La Jolla, CA., USA).

Techniques:

Journal: Toxicology

Article Title: Tumour necrosis factor-α (TNF-α) enhances dietary carcinogen-induced DNA damage in colorectal cancer epithelial cells through activation of JNK signaling pathway

doi: 10.1016/j.tox.2021.152806

Figure Lengend Snippet: Effect of TNF-α on NF-kB activation in colon cancer cells HCT116. Cells were treated with TNF-α dose range of (0.01-100 pg/mL) for 24 h. IkB and phosphorylated IkB (p-IkB) protein expression were determined by immunoblotting and quantified using Image J. Beta-actin was a loading control. Significance was assessed using one-way ANOVA (GraphPad Prism 8, (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001) compared to control. Data are presented as the ratio of p-IkB/total IkB with SEM from three independent cultures(n=3).

Article Snippet: Significant differences (P < 0.05) were determined using one-way analysis of variance (ANOVA) followed by a Dunnet post test (Graphpad Prism 8, GraphPad software Inc., La Jolla, CA., USA).

Techniques: Activation Assay, Expressing, Western Blot, Control

Journal: Toxicology

Article Title: Tumour necrosis factor-α (TNF-α) enhances dietary carcinogen-induced DNA damage in colorectal cancer epithelial cells through activation of JNK signaling pathway

doi: 10.1016/j.tox.2021.152806

Figure Lengend Snippet: Effect of TNF-α on JNK activation in colon cancer cells HCT116. Levels of phosphorylated JNK are increased upon TNF-α treatment. Cells were treated with TNF-α dose range of (0.01-100 pg/mL) for 24 h. JNK and phosphorylated JNK (p-JNK) protein expression were determined by immunoblotting and quantified using Image J. Beta-actin was a loading control. Significance was assessed using one-way ANOVA (GraphPad Prism 8, (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001) compared to control. Data are presented as the ratio of p-IkB/total IkB with SEM from three independent cultures(n=3).

Article Snippet: Significant differences (P < 0.05) were determined using one-way analysis of variance (ANOVA) followed by a Dunnet post test (Graphpad Prism 8, GraphPad software Inc., La Jolla, CA., USA).

Techniques: Activation Assay, Expressing, Western Blot, Control

Journal: Toxicology

Article Title: Tumour necrosis factor-α (TNF-α) enhances dietary carcinogen-induced DNA damage in colorectal cancer epithelial cells through activation of JNK signaling pathway

doi: 10.1016/j.tox.2021.152806

Figure Lengend Snippet: JNK and NF-kB involvement in TNF-α induced DNA damage. ( A ) genotoxicity of HCT116, cells were treated with 0.13μM of Bortezomib (BZ) with TNF-α dose range of (0.01-100 pg/mL) for 24 h. (B) genotoxicity of HCT116, cells were treated with 30μM of JNK inhibitor (SP600125) with TNF-α dose range of (0.01-100 pg/mL) for 24 h. Statistically Significant differences are shown for comparisons between inhibitor treated cells Vs. untreated cells. Significance was assessed using one-way ANOVA (GraphPad Prism 8, (**** p < 0.0001) and (++++ p < 0.0001) compared to control. Data are presented as a mean and error bars represent the SEM for independent cultures(n=3).

Article Snippet: Significant differences (P < 0.05) were determined using one-way analysis of variance (ANOVA) followed by a Dunnet post test (Graphpad Prism 8, GraphPad software Inc., La Jolla, CA., USA).

Techniques: Control